Several hours after the initial tweet, Spartak published another post with midfielder Fernando, speaking in Russian, saying that there was "no racism at Spartak, we are friends and a family".
Rocha, Fernando and Luiz Adriano are all black.
Spartak Moscow have been accused of racism after posting a video of three black players stretching during a warm-weather training session with the caption "see how the chocolate melts in the sun".
The post, featuring chocolate bar and sun emoji symbols, has since been deleted.
A later video showed Dzhikiya and the three Brazilians arm-in-arm, with the Russian global saying: "There is no racism at Spartak, we are all a big family".
It is not the first time this season Spartak have found themselves at the centre of racism claims.
"This social media post from the official account of Spartak Moscow only continues to highlight the prejudices towards black people in Russian Federation", the organisation said.
Liverpool made a formal complaint to UEFA after Brewster accused Spartak defender Leonid Mironov of abusing him during the meeting between the club's Under-19 sides.
Anti-discrimination group the FARE Network, which investigates racism issues for European soccer's governing body UEFA, criticized Spartak.
Russian Federation host the 2018 World Cup finals in June but some players have raised concerns over their suitability given that racist incidents remain commonplace in the country's domestic league.
Spartak fans were also found guilty of abusing Liverpool's Bobby Adekanye during a Youth League fixture in Russian Federation.
Russia, billed to host the FIFA World Cup in June has a long history of racial prejudices and intolerance.
"It is a reminder, that along with the whole of football, there is significant work to do to eradicate racism of all forms from the sport".