As a result of the administrative order imposing the fine, "the active regulatory offense proceedings conducted by the Munich II public prosecutor against Audi AG will be finally terminated".
Audi has been fined €800 million (around £700m) as part of a settlement with German prosecutors investigating breaches of diesel emissions rules.
The penalty by Munich prosecutors is just the latest effect of the scandal that emerged in 2015 and initially wiped out billions off the company's value.
The company said in response: "Audi accepts the fine and, by doing so, admits its responsibility".
"Audi AG has accepted the fine" investigators levied for "deviations from regulatory requirements in certain V6 and V8 diesel aggregates (motors) and diesel vehicles", the group said in a statement, adding that "the fine will directly affect Volkswagen AG's financial earnings" for 2018.
VW admitted in 2015 to building so-called "defeat devices" into 11 million cars worldwide.
The scam involved the use of software allowing vehicles to appear to be meeting regulatory emissions requirements under lab conditions, while in fact they were spewing out many times more harmful gases like nitrogen oxides (NOx) on the road.
The cheating emissions case covered around 4.9 million Audi cars sold in Europe, the United States and elsewhere between 2004 and 2018. Former chief Martin Winterkorn and other executives face criminal charges in the United States, though they can not legally be extradited.
Earlier this month, Volkswagen broke the contract of Audi Chief Executive Rupert Stadler, who is now being investigated over his alleged involvement in the diesel cheating.
Prosecutors had jailed him in June, saying the move was necessary to stop him trying to influence witnesses.