The Barksdales are charged in the Arizona murder of Frank Bligh, 72, whose body has not been found.
The Navajo County, Arizona, sheriff's office said in a statement that the Barksdales were arrested without incident and that the sheriff's office assisted the marshals service in the capture.
'It was compelling enough that they felt the need to pull over, ' David Gonzales, the US marshal for Arizona, said of Susan's so-called medical emergency.
The Marshals Service was offering rewards of $25,000 for information leading to the arrest of Blane and $10,000 for Susan.
Blane Barksdale and his wife Susan, both wanted for murder, escaped custody last month by overpowering a transport team that was taking them from upstate NY to Pima County, Arizona. After ditching the van, the couple drove off in the pickup truck, according to Gonzales.
Tucson authorities said the Barksdales might have been getting help to stay in hiding.
They were considered armed and risky after breaking free from two guards who were taking them from upstate NY to Tucson, Arizona to face murder charges over the killing of an elderly Vietnam veteran and burning of his house last April. They don't think the couple made it to Mexico, KOLD says.
Gonzales previously told The Associated Press that after Susan Barksdale, 59, faked the medical emergency, the guards pulled over the transport van and were overpowered by the couple as soon as they opened the back compartment. They then tied up the guards as well as a third inmate who was in the van, said David Gonzales, U.S. Marshal for the District of Arizona, at a news conference Monday.
The two homicide suspects who escaped police during transport from Rochester to Arizona have been captured.
From there, the Barksdales drove the van into Arizona. His house was set on fire and his auto was stolen in April.
But he and his vehicle were nowhere to be found, and investigators discovered evidence the fire was set intentionally and that Bligh had been killed, Tucson police said.